Harry P. Rappaport

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Office: Department of Biology

450 Biological Life Sciences Building
Temple University
Philadelphia, PA 19122

Phone: (215) 204-8875
E-mail: oldman@temple.edu

Dr. Rappaport has a program to determine the physical possibility of expanding the number of types of base pairs in DNA. The pairing stabilities of the analog bases 6-thioguanine and 5-methyl-2-pyrimidinone were measured. Procedures were developed to synthesize oligodeoxynucleotides with the analog bases. An enzymatic procedure was developed to measure relative association constants of oligomer. Initial studies indicated the stability of the synthetic base pair is the same as the natural base pair adenine/thymine. Presently the replication of the base pair in DNA is under investigation using synthetic oligonucleotides as primers and templates. Sample papers: "Replication of the base pair 6-thioguanine/5-methyl-2-pyrimidinone." Biochemistry 32: 3047-3057: 1993., "The fidelity of replication of the three base pairs hypoxanthine/cyctosine, adenine/thymine, and 6-thiopurine/5-methyl-2-pyrimidinone with T7 DNA polymerase,(2004), Biochem J.,709-717